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Dot plot of PCA based on fibroblasts-specific ( A ) and endothelial cell-specific ( B ) gene markers to estimate their ability in distinguish the LNs subtypes ( n = 68). C Box plots illustrating the differences in <t>COL1A1,</t> α-SMA, FN1, CD31, and LYVE1 gene expression between LNs subtypes ( n = 50, 35, 31, 32, 11, and 14). Each data point represents an individual LN (biological replicates). Box plots represent the median (center line), 25th and 75th percentiles (bounds of the box), and minimum and maximum values (whiskers). The mIF staining and quantification reveal differential expression of α-SMA, FN1, COL1A1 ( n = 20 and 16) ( D ) and LYVE-1, CD31 ( n = 24 and 18) ( E ) between NLN_C1 and NLN_C4. Each data point represents an individual LN (biological replicates). Data are presented as mean values ± SD. The p value was calculated using a two-sided Student’s t test. Scale bars indicate 100 μm, 500 μm, and 1000 μm respectively. Source data are provided as a Source Data file. F Sirius Red staining results of differences in fibrosis levels between NLN_C1 and NLN_C4. Scale bars indicate 1000 μm. PC principal component, PCA principal component analysis, mIF multiplex immunofluorescence, SD standard deviation.
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Dot plot of PCA based on fibroblasts-specific ( A ) and endothelial cell-specific ( B ) gene markers to estimate their ability in distinguish the LNs subtypes ( n = 68). C Box plots illustrating the differences in <t>COL1A1,</t> α-SMA, FN1, CD31, and LYVE1 gene expression between LNs subtypes ( n = 50, 35, 31, 32, 11, and 14). Each data point represents an individual LN (biological replicates). Box plots represent the median (center line), 25th and 75th percentiles (bounds of the box), and minimum and maximum values (whiskers). The mIF staining and quantification reveal differential expression of α-SMA, FN1, COL1A1 ( n = 20 and 16) ( D ) and LYVE-1, CD31 ( n = 24 and 18) ( E ) between NLN_C1 and NLN_C4. Each data point represents an individual LN (biological replicates). Data are presented as mean values ± SD. The p value was calculated using a two-sided Student’s t test. Scale bars indicate 100 μm, 500 μm, and 1000 μm respectively. Source data are provided as a Source Data file. F Sirius Red staining results of differences in fibrosis levels between NLN_C1 and NLN_C4. Scale bars indicate 1000 μm. PC principal component, PCA principal component analysis, mIF multiplex immunofluorescence, SD standard deviation.
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Developmental Studies Hybridoma Bank mouse anti collagen type ii monoclonal antibody 24
Dot plot of PCA based on fibroblasts-specific ( A ) and endothelial cell-specific ( B ) gene markers to estimate their ability in distinguish the LNs subtypes ( n = 68). C Box plots illustrating the differences in <t>COL1A1,</t> α-SMA, FN1, CD31, and LYVE1 gene expression between LNs subtypes ( n = 50, 35, 31, 32, 11, and 14). Each data point represents an individual LN (biological replicates). Box plots represent the median (center line), 25th and 75th percentiles (bounds of the box), and minimum and maximum values (whiskers). The mIF staining and quantification reveal differential expression of α-SMA, FN1, COL1A1 ( n = 20 and 16) ( D ) and LYVE-1, CD31 ( n = 24 and 18) ( E ) between NLN_C1 and NLN_C4. Each data point represents an individual LN (biological replicates). Data are presented as mean values ± SD. The p value was calculated using a two-sided Student’s t test. Scale bars indicate 100 μm, 500 μm, and 1000 μm respectively. Source data are provided as a Source Data file. F Sirius Red staining results of differences in fibrosis levels between NLN_C1 and NLN_C4. Scale bars indicate 1000 μm. PC principal component, PCA principal component analysis, mIF multiplex immunofluorescence, SD standard deviation.
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Image Search Results


Dot plot of PCA based on fibroblasts-specific ( A ) and endothelial cell-specific ( B ) gene markers to estimate their ability in distinguish the LNs subtypes ( n = 68). C Box plots illustrating the differences in COL1A1, α-SMA, FN1, CD31, and LYVE1 gene expression between LNs subtypes ( n = 50, 35, 31, 32, 11, and 14). Each data point represents an individual LN (biological replicates). Box plots represent the median (center line), 25th and 75th percentiles (bounds of the box), and minimum and maximum values (whiskers). The mIF staining and quantification reveal differential expression of α-SMA, FN1, COL1A1 ( n = 20 and 16) ( D ) and LYVE-1, CD31 ( n = 24 and 18) ( E ) between NLN_C1 and NLN_C4. Each data point represents an individual LN (biological replicates). Data are presented as mean values ± SD. The p value was calculated using a two-sided Student’s t test. Scale bars indicate 100 μm, 500 μm, and 1000 μm respectively. Source data are provided as a Source Data file. F Sirius Red staining results of differences in fibrosis levels between NLN_C1 and NLN_C4. Scale bars indicate 1000 μm. PC principal component, PCA principal component analysis, mIF multiplex immunofluorescence, SD standard deviation.

Journal: Nature Communications

Article Title: Lymph nodes molecular subtypes unravel lymph nodes heterogeneity and clinical implications in colorectal cancer

doi: 10.1038/s41467-025-63200-z

Figure Lengend Snippet: Dot plot of PCA based on fibroblasts-specific ( A ) and endothelial cell-specific ( B ) gene markers to estimate their ability in distinguish the LNs subtypes ( n = 68). C Box plots illustrating the differences in COL1A1, α-SMA, FN1, CD31, and LYVE1 gene expression between LNs subtypes ( n = 50, 35, 31, 32, 11, and 14). Each data point represents an individual LN (biological replicates). Box plots represent the median (center line), 25th and 75th percentiles (bounds of the box), and minimum and maximum values (whiskers). The mIF staining and quantification reveal differential expression of α-SMA, FN1, COL1A1 ( n = 20 and 16) ( D ) and LYVE-1, CD31 ( n = 24 and 18) ( E ) between NLN_C1 and NLN_C4. Each data point represents an individual LN (biological replicates). Data are presented as mean values ± SD. The p value was calculated using a two-sided Student’s t test. Scale bars indicate 100 μm, 500 μm, and 1000 μm respectively. Source data are provided as a Source Data file. F Sirius Red staining results of differences in fibrosis levels between NLN_C1 and NLN_C4. Scale bars indicate 1000 μm. PC principal component, PCA principal component analysis, mIF multiplex immunofluorescence, SD standard deviation.

Article Snippet: The primary antibodies included rabbit polyclonal antibody CXCL13/BCA1 (Proteintech, 10927-1-AP); rabbit polyclonal antibody LYVE1 (Proteintech, 28321-1-AP); mouse monoclonal antibody CD20 (Proteintech, 60271-1-Ig); mouse monoclonal antibody FN1 (Proteintech, 66042-1-Ig); mouse monoclonal antibody CD31 (Proteintech, 66065-2-Ig); mouse monoclonal antibody COL1A1 (Proteintech, 67288-1-Ig); mouse monoclonal antibody PDPN (Proteintech, 67432-1-Ig); mouse monoclonal antibody CD35 (Proteintech, 68033-1-Ig); rabbit recombinant antibody α-SMA (Proteintech, 80008-1-RR); All images were captured using an EVOS FL Auto 2 Imaging System (Thermo Fisher Scientific).

Techniques: Gene Expression, Staining, Quantitative Proteomics, Multiplex Assay, Immunofluorescence, Standard Deviation